Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody [BLR260L]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a recombinant protein representing the extracellular domain of mouse Thrombomodulin (residues 17-517, NP_033404.1).
Additional Product Information
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P07204].
Alternate Names
AI385582; CD antigen CD141; CD141; fetomodulin; snoRNA MBII-339; thrombomodulin; TM
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.