Rabbit anti-TIAR Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-613A maps to a region between residue 325 and 375 of human TIA-1-Related Protein using the numbering given in entry NP_003243.1 (GeneID 7073).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TIAR immobilized on solid support.
The epitope recognized by A303-613A-T maps to a region between residue 325 and 375 of human TIA-1-Related Protein using the numbering given in entry NP_003243.1 (GeneID 7073).
Additional Product Information
TIA-1 related protein (TIAR) is an RNA-binding protein. TIAR is also called nucleolysin and possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIAR may be involved in apoptosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q01085].
Alternate Names
aging-associated gene 7 protein; nucleolysin TIAR; TCBP; T-cluster binding protein; TIA-1-related nucleolysin; TIA-1-related protein; TIAR
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.