Rabbit anti-TIGAR Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-286A maps to a region between residue 220 and 270 of human TP53-induced glycolysis and apoptosis regulator using the numbering given in entry NP_065108.1 (GeneID 57103).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TIGAR immobilized on solid support.
The epitope recognized by A302-286A-T maps to a region between residue 220 and 270 of human TP53-induced glycolysis and apoptosis regulator using the numbering given in entry NP_065108.1 (GeneID 57103).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TIGAR (TP53-induced glycolysis and apoptosis regulator) is a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis [taken from NCBI GeneID:57103].
Alternate Names
C12orf5; FR2BP; fructose-2,6-bisphosphatase TIGAR; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase; transactivated by NS3TP2 protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.