Rabbit anti-Timeless Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-961A maps to a region between residue 1170 and the C-terminus (residue 1208) of human Timeless using the numbering given in entry NP_003911.1 (GeneID 8914).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Timeless immobilized on solid support.
The epitope recognized by A300-961A-T maps to a region between residue 1170 and the C-terminus (residue 1208) of human Timeless using the numbering given in entry NP_003911.1 (GeneID 8914).
Additional Product Information
Timeless was first identified in Drosophila as a transcriptional regulator involved in the fly circadian rhythm. In mice, humans and yeast, the homologs of drosophila timeless have been observed to play a role in the DNA damage checkpoint response. Human timeless has been shown to interact with the circadian clock protein cryptochrome 2 as well as the DNA checkpoint proteins Chk1 and the ATR-ATRIP complex suggesting a link between circadian rhythm and the cell cycle.
Alternate Names
hTIM; protein timeless homolog; TIM; TIM1; timeless circadian clock 1; timeless homolog; Tof1 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.