Rabbit anti-TLN1/Talin-1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-206A maps to a region between residue 1875 to 1925 of human Talin-1 using the numbering given in entry Q9Y490.3 (GeneID 7094).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TLN1 immobilized on solid support.
The epitope recognized by A305-206A-T maps to a region between residue 1875 to 1925 of human Talin-1 using the numbering given in entry Q9Y490.3 (GeneID 7094).
Additional Product Information
This gene TLN1 encodes for the cytoskeletal protein Talin-1. This protein is concentrated in areas of cell-substratum and cell-cell contacts. Talin-1 plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells [taken from NCBI Entrez Gene (Gene ID: 7094)].
Alternate Names
ILWEQ; talin-1; TLN
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.