Rabbit anti-TMX4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-724A maps to a region between residue 299 to 349 of human Thioredoxin-related transmembrane protein 4 using the numbering given in entry Q9H1E5.1 (GeneID 56255).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TMX4 immobilized on solid support.
The epitope recognized by A305-724A-T maps to a region between residue 299 to 349 of human Thioredoxin-related transmembrane protein 4 using the numbering given in entry Q9H1E5.1 (GeneID 56255).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TMX4 is a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. TMX4 has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, one transmembrane domain and C-terminal ASP/GLU-rich calcium binding domain. Unlike most members of this family, it lacks a C-terminal ER-retention sequence. TMX4 has been shown to have reductase activity in vitro. [provided by RefSeq, Jan 2017] [taken from NCBI Entrez Gene (Gene ID: 56255)]. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H1E5].
Alternate Names
DJ971N18.2; PDIA14; protein disulfide isomerase family A, member 14; thioredoxin domain containing 13; thioredoxin domain-containing protein 13; thioredoxin-related transmembrane protein 4; TXNDC13
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.