Rabbit anti-TNF-alpha Recombinant Monoclonal Antibody [BLR330M]
Product Details
Specifications
Immunogen was a recombinant protein representing the extracellular domain of human Tumor Necrosis Factor Alpha (residues 77-233, NP_000585.2).
Additional Product Information
TNF-alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF-alpha can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-alpha has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. [taken from NCBI Entrez Gene (Gene ID: 7124)].
Alternate Names
APC1 protein;cachectin;DIF;ligand superfamily member 2;TNF, macrophage-derived;TNF, monocyte-derived;TNFA;TNF-a;TNF-alpha;TNFSF2;TNLG1F;tumor necrosis factor;tumor necrosis factor ligand 1F;tumor necrosis factor ligand superfamily member 2;tumor necrosis factor-alpha
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.