Rabbit anti-TOE1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-643A maps to a region between residue 460 and 510 of human Target of EGR1, Member 1 using the numbering given in entry NP_079353.3 (GeneID 114034).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TOE1 immobilized on solid support.
The epitope recognized by A303-643A-T maps to a region between residue 460 and 510 of human Target of EGR1, Member 1 using the numbering given in entry NP_079353.3 (GeneID 114034).
Additional Product Information
Target of EGR1 protein 1 (TOE1) inhibits cell growth rate and cell cycle. It induces CDKN1A expression as well as TGF-beta expression. TOE1 also mediates the inhibitory growth effect of EGR1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96GM8].
Alternate Names
hCaf1; ZPCH7; target of EGR1 protein 1; target of EGR1, member 1 (nuclear)
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.