Rabbit anti-TOP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-589A maps to a region between residue 100 and 150 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TOP1 immobilized on solid support.
The epitope recognized by A302-589A-T maps to a region between residue 100 and 150 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).
Additional Product Information
Topoisomerase I (TOPI) functions as an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA [taken from NCBI Entrez Gene (GeneID: 7150)].
Alternate Names
DNA topoisomerase 1; DNA topoisomerase I; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.