Rabbit anti-TPX2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-429A maps to a region between residues 150 and 200 of human TPX2 using the numbering given in Swiss-Prot entry Q9ULW0 (GeneID 22974).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TPX2 immobilized on solid support.
The epitope recognized by A300-429A-T maps to a region between residues 150 and 200 of human TPX2 using the numbering given in Swiss-Prot entry Q9ULW0 (GeneID 22974).
Additional Product Information
TPX2 is a microtubule associated protein (MAP) that co-localizes with spindle microtubules during M-phase of the cell cycle. It may function to nucleate microtubules and initiate microtubule polymerization and assembly for spindle formation. TPX2 is a target of RanGTP, a small GTPase that coordinates spindle assembly. TPX2 protein levels peak during mitosis and are tightly regulated by ubiquitination during the cell cycle.
Alternate Names
C20orf1; C20orf2; differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; DIL2; DIL-2; FLS353; GD:C20orf1; HCA519; HCTP4; hepatocellular carcinoma-associated antigen 519; hepatocellular carcinoma-associated antigen 90; p100; preferentially expressed in colorectal cancer; protein fls353; REPP86; restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; targeting protein for Xklp2; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.