Rabbit anti-TRAP100/MED24 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-472A maps to a region between residue 939 and 989 of human thyroid hormone receptor-associated protein complex 100 kDa component (mediator complex subunit 24 ) using the numbering given in entry NP_055630.2 (GeneID 9862).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TRAP100/MED24 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. TRAP100/MED24 associates with mediator subunit TRAP220. Both are essential to mouse development and play a role in androgen receptor-mediated transcription. Studies in zebrafish have shown that TRAP100/MED24 is also important to retinal development.
Alternate Names
activator-recruited cofactor 100 kDa component; ARC100; cofactor required for Sp1 transcriptional activation subunit 4; cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa; CRSP complex subunit 4; CRSP100; CRSP4; DRIP100; hTRAP100; MED5; Mediator complex subunit 24; mediator of RNA polymerase II transcription subunit 24; mediator of RNA polymerase II transcription, subunit 24 homolog; THRAP4; thyroid hormone receptor-associated protein 4; thyroid hormone receptor-associated protein complex 100 kDa component; TRAP100; vitamin D3 receptor-interacting protein complex 100 kDa component; vitamin D3 receptor-interacting protein complex component DRIP100
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.