Rabbit anti-TRIM3/BERP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-207A-T maps to a region between residue 1 and 50 of human tripartite motif-containing 3 (brain expressed ring finger protein) using the numbering given in entry NP_006449.2 (GeneID 10612).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TRIM3/BERP immobilized on solid support.
The epitope recognized by A301-207A maps to a region between residue 1 and 50 of human tripartite motif-containing 3 (brain expressed ring finger protein) using the numbering given in entry NP_006449.2 (GeneID 10612).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TRIM3/BERP is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM3/BERP has been identified as a component of the CART (cytoskeleton-associated recycling or transport) complex which includes actinin-4, hrs, myosin V, and TRIM3/BERP. In this complex TRIM/BERP is involved in the recycling of plasma membrane receptors.
Alternate Names
BERP; brain expressed ring finger; brain-expressed RING finger protein; HAC1; ring finger protein 22; RING finger protein 97; RNF22; RNF97; tripartite motif protein TRIM3; tripartite motif-containing protein 3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.