Rabbit anti-TRM6 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-008A maps to a region between residue 447 and 497 of human tRNA Methyltransferase 6 using the numbering given in entry NP_057023.2 (GeneID 51605).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TRM6 immobilized on solid support.
The epitope recognized by A303-008A-T maps to a region between residue 447 and 497 of human tRNA Methyltransferase 6 using the numbering given in entry NP_057023.2 (GeneID 51605).
Additional Product Information
tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6 is a substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UJA5].
Alternate Names
CGI-09; GCD10; Gcd10p; mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6; tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6; tRNA methyltransferase 6 homolog; tRNA(m1A58)-methyltransferase subunit TRM6; tRNA(m1A58)MTase subunit TRM6
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.