Rabbit anti-TRMT1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-205A-T maps to a region between residue 609 to 659 of human TRM1 tRNA Methyltransferase 1 Homolog using the numbering given in entry NP_060192.1 (GeneID 55621).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TRMT1 immobilized on solid support.
The epitope recognized by A304-205A maps to a region between residue 609 to 659 of human TRM1 tRNA Methyltransferase 1 Homolog using the numbering given in entry NP_060192.1 (GeneID 55621).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
tRNA 2,2-dimethylguanosine-26 methyltransferas (TRMT1) dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NXH9].
Alternate Names
N(2),N(2)-dimethylguanosine tRNA methyltransferase; TRM1; TRM1 tRNA methyltransferase 1 homolog; tRNA (guanine(26)-N(2))-dimethyltransferase; tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA methyltransferase 1 homolog; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.