Rabbit anti-TSC1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-316A maps to a region between residue 1100 and the C-terminus (residue 1164) of human tuberous sclerosis 1 using the numbering given in Swiss-Prot entry Q92574 (GeneID 7248).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TSC1 immobilized on solid support.
The epitope recognized by A300-316A-T maps to a region between residue 1100 and the C-terminus (residue 1164) of human tuberous sclerosis 1 using the numbering given in Swiss-Prot entry Q92574 (GeneID 7248).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Tuberous sclerosis 1 protein (TSC1) forms a heterodimer with TSC2 to integrate cellular growth and stress signals. Defects in the TSC1 and TSC2 gene are responsible for the genetic disorder tuberous sclerosis. The TSC1/TSC2 complex functions in the mTOR pathway by negatively regulating mTOR activity via the small GTP-binding protein Rheb.
Alternate Names
hamartin; LAM; truncated hemartin; TSC; tuberous sclerosis 1 protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.