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Rabbit anti-TWIST Recombinant Monoclonal Antibody [BLR156J]

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
TWIST1
Reactivity:
Human
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
BLR156J
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Purified
100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
250 µg/ml 1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Country of Origin
USA

Additional Product Information

TWIST1 is a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. TWIST1 forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. TWIST1 may also regulate neural tube closure, limb development and brown fat metabolism. The TWIST1 gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. [taken from NCBI Entrez Gene (Gene ID: 7291)].

7291
TWIST1
twist family bHLH transcription factor 1
Q15672
Twist-related protein 1

Alternate Names

ACS3; B-HLH DNA binding protein; bHLHa38; BPES2; BPES3; class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; SCS; SWCOS; TWIST; twist basic helix-loop-helix transcription factor 1; twist homolog 1; TWIST homolog of drosophila; twist-related protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.