Rabbit anti-UBAC1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-120A-T maps to a region between residue 100 to 150 of human UBA Domain Containing 1 using the numbering given in entry NP_057256.2 (GeneID 10422).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBAC1 immobilized on solid support.
The epitope recognized by A304-120A maps to a region between residue 100 to 150 of human UBA Domain Containing 1 using the numbering given in entry NP_057256.2 (GeneID 10422).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
UBA domain containing 1 (UBAC1) is a non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. UBAC1 is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BSL1].
Alternate Names
E3 ubiquitin-protein ligase subunit KPC2; GBDR1; glialblastoma cell differentiation-related protein 1; kip1 ubiquitination-promoting complex protein 2; Kip1 ubiquitination-promoting complex subunit 2; KPC2; putative glialblastoma cell differentiation-related protein; testicular secretory protein Li 64; UBA domain-containing protein 1; UBADC1; ubiquitin associated domain containing 1; ubiquitin-associated domain-containing protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.