Rabbit anti-UBAP2L/NICE4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-533A maps to a region between residues 400 and 450 of human Ubiquitin Associated Protein 2-Like using the numbering given in entry AAH03170.1 (GeneID 9898). The epitope is also found in the shorter entry BAA09765.1. The more quickly migrating protein detected by using A300-533A (but not detected by A300-534A) is believed to be the shorter from of UBAP2L/NICE4.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBAP2L/NICE4 immobilized on solid support.
The epitope recognized by A300-533A-T maps to a region between residues 400 and 450 of human Ubiquitin Associated Protein 2-Like using the numbering given in entry AAH03170.1 (GeneID 9898). The epitope is also found in the shorter entry BAA09765.1. The more quickly migrating protein detected by using A300-533A-T (but not detected by A300-534A) is believed to be the shorter from of UBAP2L/NICE4.
Additional Product Information
Ubiquitin associated protein 2-like (UBAP2L) has been identified as the predicted gene product of a coding sequence deduced by analysis of cDNAs from a human myeloid cell line. UBAP2L was also identified as a cDNA belonging to the epidermal differentiation complex (EDC) which comprises a large number of genes important to human epidermis maturation. A function of UBAP2L has not been assigned.
Alternate Names
NICE4; NICE-4; protein NICE-4; ubiquitin-associated protein 2-like
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.