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Rabbit anti-UBE2E2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
UBE2E2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to UBE2E2 immobilized on solid support.

The epitope recognized by A303-485A maps to a region between residue 25 and 75 of human Ubiquitin-conjugating Enzyme E2E 2 using the numbering given in entry NP_689866.1 (GeneID 7325).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to UBE2E2 immobilized on solid support.

The epitope recognized by A303-485A-T maps to a region between residue 25 and 75 of human Ubiquitin-conjugating Enzyme E2E 2 using the numbering given in entry NP_689866.1 (GeneID 7325).
Country of Origin
USA

Additional Product Information

ubiquitin-conjugating enzyme E2 E2 (UBE2E2) is a ubiquitin conjugating enzyme.

7325
UBE2E2
ubiquitin conjugating enzyme E2 E2
Q96LR5
Ubiquitin-conjugating enzyme E2 E2

Alternate Names

E2 ubiquitin-conjugating enzyme E2; UBCH8; ubiquitin carrier protein E2; ubiquitin conjugating enzyme E2E 2; ubiquitin-conjugating enzyme E2 E2; ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast); ubiquitin-protein ligase E2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.