Rabbit anti-UBE2E2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-485A maps to a region between residue 25 and 75 of human Ubiquitin-conjugating Enzyme E2E 2 using the numbering given in entry NP_689866.1 (GeneID 7325).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBE2E2 immobilized on solid support.
The epitope recognized by A303-485A-T maps to a region between residue 25 and 75 of human Ubiquitin-conjugating Enzyme E2E 2 using the numbering given in entry NP_689866.1 (GeneID 7325).
Additional Product Information
ubiquitin-conjugating enzyme E2 E2 (UBE2E2) is a ubiquitin conjugating enzyme.
Alternate Names
E2 ubiquitin-conjugating enzyme E2; UBCH8; ubiquitin carrier protein E2; ubiquitin conjugating enzyme E2E 2; ubiquitin-conjugating enzyme E2 E2; ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast); ubiquitin-protein ligase E2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.