Rabbit anti-UBF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-859A-T maps to a region between residue 546 and 596 of human upstream binding transcription factor, RNA polymerase I using the numbering given in entry NP_055048.1 (GeneID 7343).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBF immobilized on solid support.
The epitope recognized by A301-859A maps to a region between residue 546 and 596 of human upstream binding transcription factor, RNA polymerase I using the numbering given in entry NP_055048.1 (GeneID 7343).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Upstream binding factor (UBF) was originally identified as a multi-HMG (high mobility group)-box DNA binding domain protein that could enhance the in vitro assembly of the RNA polymerase I initiation complex. UBF is a transcription factor that recognizes the ribosomal RNA gene promoter required for the expression of 18S, 5.8S, and 28S ribosomal RNAs.
Alternate Names
90-kDa nucleolus organizer region autoantigen; autoantigen NOR-90; CONDBA; NOR-90; nucleolar transcription factor 1; UBF; UBF1; UBF-1; UBF2; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.