Rabbit anti-UBR2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-416A maps to a region between residue 1705 to 1755 of human E3 ubiquitin-protein ligase UBR2 using the numbering given in entry Q8IWV8.1 (GeneID 23304).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBR2 immobilized on solid support.
The epitope recognized by A305-416A-T maps to a region between residue 1705 to 1755 of human E3 ubiquitin-protein ligase UBR2 using the numbering given in entry Q8IWV8.1 (GeneID 23304).
Additional Product Information
UBR2 is an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation [taken from NCBI Entrez Gene (Gene ID: 23304)].
Alternate Names
bA49A4.1; C6orf133; dJ242G1.1; dJ392M17.3; E3 ubiquitin-protein ligase UBR2; N-recognin-2; RING-type E3 ubiquitin transferase UBR2; ubiquitin-protein ligase E3-alpha-2; ubiquitin-protein ligase E3-alpha-II
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.