Rabbit anti-UBXN1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-415A-T maps to a region between residue 50 and 100 of human UBX Domain Protein 1 using the numbering given in entry NP_056937.2 (GeneID 51035). Antibody was affinity purified using an epitope specific to UBXN1 immobilized on solid support.
The epitope recognized by A303-415A maps to a region between residue 50 and 100 of human UBX Domain Protein 1 using the numbering given in entry NP_056937.2 (GeneID 51035).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
UBX domain-containing protein 1 (UBXN1) is a ubiquitin-binding protein that interacts with the BRCA1-BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. The interaction between UBXN1 and autoubiquitinated BRCA1, leads to the inhibition the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. UBXN1 is also a component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol.3 kDa protein, UBXD10, 2B28, and SAKS1.
Alternate Names
2B28; SAKS1; SAPK substrate protein 1; UBA/UBX 33.3 kDa protein; UBX domain-containing protein 1; UBXD10
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.