Rabbit anti-UQCRC2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-414A maps to a region between residue 1 to 50 of human Cytochrome b-c1 complex subunit 2, mitochondrial using the numbering given in entry P22695.3 (GeneID 7385).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UQCRC2 immobilized on solid support.
The epitope recognized by A305-414A-T maps to a region between residue 1 to 50 of human Cytochrome b-c1 complex subunit 2, mitochondrial using the numbering given in entry P22695.3 (GeneID 7385).
Additional Product Information
Ubiquinol-cytochrome-c reductase complex core protein 2 (UQCRC2) is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P22695].
Alternate Names
complex III subunit 2; Core protein II; cytochrome bc-1 complex core protein II; cytochrome b-c1 complex subunit 2, mitochondrial; MC3DN5; QCR2; ubiquinol-cytochrome c reductase core protein II; ubiquinol-cytochrome-c reductase complex core protein 2; UQCR2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.