Rabbit anti-USP14 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-919A maps to a region between residue 350 and 400 of human Ubiquitin Specific Peptidase 14 using the numbering given in entry NP_005142.1 (GeneID 9097).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to USP14 immobilized on solid support.
The epitope recognized by A300-919A-T maps to a region between residue 350 and 400 of human Ubiquitin Specific Peptidase 14 using the numbering given in entry NP_005142.1 (GeneID 9097).
Additional Product Information
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 14 (USP14) is a member of this family.
Alternate Names
deubiquitinating enzyme 14; TGT; tRNA-guanine transglycosylase, 60-kD subunit; ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14 (tRNA-guanine transglycosylase); ubiquitin thioesterase 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; Ubiquitin-specific-processing protease 14
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.