Rabbit anti-USP5/IsoT IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00313 maps to a region between residue 375 and 425 of human ubiquitin specific peptidase 5 (isopeptidase T) using the numbering given in entry CAA62690.1 (GeneID 8078).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to USP5/IsoT immobilized on solid support.
The epitope recognized by IHC-00313-T maps to a region between residue 375 and 425 of human ubiquitin specific peptidase 5 (isopeptidase T) using the numbering given in entry CAA62690.1 (GeneID 8078).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome.
Alternate Names
deubiquitinating enzyme 5; isopeptidase T; ISOT; testicular tissue protein Li 218; ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific protease 5 (isopeptidase T); ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ubiquitin-specific-processing protease 5
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.