Rabbit anti-UTP18/WDR50 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-552A-T maps to a region between residue 325 and 375 of human small subunit (SSU) processome component UTP18 (WD repeat domain 50) using the numbering given in entry NP_057085.2 (GeneID 51096).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UTP18/WDR50 immobilized on solid support.
The epitope recognized by A301-552A maps to a region between residue 325 and 375 of human small subunit (SSU) processome component UTP18 (WD repeat domain 50) using the numbering given in entry NP_057085.2 (GeneID 51096).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
UTP18/WDR50 is a component of the small-subunit (SSU) processome, a large ribonucleoprotein complex that participates in ribosomal RNA processing and ribosome assembly. UTP18/WDR50 is a member of the WD repeat family. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.
Alternate Names
CGI-48; U3 small nucleolar RNA-associated protein 18 homolog; UTP18, small subunit (SSU) processome component, homolog; WD repeat domain 50; WD repeat-containing protein 50; WDR50
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.