Rabbit anti-VAV3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-833A maps to a region between residue 200 and 250 of human vav 3 guanine nucleotide exchange factor using the numbering given in entry NP_006104.4 (GeneID 10451).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Vav3 is a member of the VAV guanine nucleotide exchange factor (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. Vav3 acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases [taken from NCBI Entrez Gene (Gene ID: 10451)].
Alternate Names
guanine nucleotide exchange factor VAV3; vav 3 guanine nucleotide exchange factor; vav 3 oncogene; VAV-3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.