Rabbit anti-Visfatin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-779A maps to a region between residue 450 and the C-terminus (residue 491) of human Visfatin (PBEF/NAMPT) using the numbering given in entry NP_005737.1 (GeneID 10135).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Visfatin immobilized on solid support.
The epitope recognized by A300-779A-T maps to a region between residue 450 and the C-terminus (residue 491) of human Visfatin (PBEF/NAMPT) using the numbering given in entry NP_005737.1 (GeneID 10135).
Additional Product Information
PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.
Alternate Names
1110035O14Rik; NAmPRTase; Nampt; nicotinamide phosphoribosyltransferase; PBEF; PBEF1; pre-B cell-enhancing factor; pre-B-cell colony-enhancing factor 1; VF; VISFATIN
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.