Rabbit anti-XLF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-729A-T maps to a region between residue 200 and 250 of human XRCC4-Like Factor using the numbering given in entry NP_079058.1 (GeneID 79840).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to XLF immobilized on solid support.
The epitope recognized by A300-729A maps to a region between residue 200 and 250 of human XRCC4-Like Factor using the numbering given in entry NP_079058.1 (GeneID 79840).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
XRCC4-like factor (XLF) functions in DNA nonhomologous end joining (NHEJ) in double-strand break repair and immune system V(D)J recombination. Mutations of XLF in humans have been documented to result in immunodeficiency, growth retardation, and microcephaly. The yeast of homolog of XLF is Nej1. XLF has been shown to interact with the XRCC4 x DNA-ligase IV complex and is implicated in the DNA religation step of the NHEJ pathway.
Alternate Names
non-homologous end-joining factor 1; nonhomologous end-joining factor 1; protein cernunnos; XLF; XRCC4-like factor
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.