Rabbit anti-YARS/Tyrosyl-tRNA Synthetase/TYRRS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-064A maps to a region between residue 1 to 50 of human Tyrosyl-tRNA synthetase, cytoplasmic using the numbering given in entry P54577.4 (GeneID 8565).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to YARS/Tyrosyl-tRNA Synthetase/TYRRS immobilized on solid support.
The epitope recognized by A305-064A-T maps to a region between residue 1 to 50 of human Tyrosyl-tRNA synthetase, cytoplasmic using the numbering given in entry P54577.4 (GeneID 8565).
Additional Product Information
The YARS gene encodes the protein Tyrosine--tRNA ligase, cytoplasmic, also known as Tyrosyl-tRNA synthetase. Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution [taken from NCBI Entrez Gene (Gene ID: 8565)].
Alternate Names
CMTDIC; tyrosine tRNA ligase 1, cytoplasmic; tyrosine--tRNA ligase, cytoplasmic; tyrosyl--tRNA ligase; Tyrosyl-tRNA synthetase; tyrosyl-tRNA synthetase, cytoplasmic; TYRRS; YRS; YTS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.