Rabbit anti-ZDHHC5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-652A maps to a region between residue 500 to 550 of human Zinc Finger, DHHC-Type Containing 5 using the numbering given in entry NP_056272.2 (GeneID 25921).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ZDHHC5 immobilized on solid support.
The epitope recognized by A304-652A-T maps to a region between residue 500 to 550 of human Zinc Finger, DHHC-Type Containing 5 using the numbering given in entry NP_056272.2 (GeneID 25921).
Additional Product Information
Zinc finger DHHC domain-containing protein 5 (ZDHHC5) is a palmitoyl acyltransferase for the G-protein coupled receptor SSTR5. ZDHHC5 also palmitoylates FLOT2 (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9C0B5].
Alternate Names
DHHC5; DHHC-5; membrane-associated DHHC5 zinc finger protein; palmitoyltransferase ZDHHC5; probable palmitoyltransferase ZDHHC5; zinc finger DHHC domain-containing protein 5; zinc finger protein 375; zinc finger, DHHC domain containing 5; ZNF375
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.