Rabbit anti-ZFP106 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-527A-T maps to a region between residue 1833 and 1883 of human zinc finger protein 106 using the numbering given in entry NP_071918.1 (GeneID 64397). Antibody was affinity purified using an epitope specific to ZFP106 immobilized on solid support.
The epitope recognized by A301-527A maps to a region between residue 1833 and 1883 of human zinc finger protein 106 using the numbering given in entry NP_071918.1 (GeneID 64397).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Zinc finger protein homolog 106 (ZFP106) is a C2H2-type zinc finger protein that also contains 2 WD repeats. ZFP106 is highly expressed in striated muscle, brown fat, and developing brain and is regulated by myogenin and nuclear respiratory factor-1 (NRF-1). ZFP106 localizes to the nucleus in association with nucleolar transcriptional machinery. The WD40 repeat in ZFP106 appears to facilitate targeting of ZFP106 to the nucleolus and to mediate the interaction between ZFP106 and testis-specific gene 118 (TSF118). A two-hybrid screen has identified testis-specific Y-encoded-like protein (TSPYL) as a ZFP106 interacting protein. These data suggest an important role for ZFP106 in testis development.
Alternate Names
SH3BP3; SH3-domain binding protein 3; ZFP106; Zfp-106; zinc finger protein 106; zinc finger protein 474; ZNF474
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.