Rabbit anti-ZNRF2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-312A maps to a region between residue 192 and 242 of human zinc and ring finger 2 using the numbering given in entry NP_667339.1 (GeneID 223082).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ZNRF2 immobilized on solid support.
The epitope recognized by A302-312A-T maps to a region between residue 192 and 242 of human zinc and ring finger 2 using the numbering given in entry NP_667339.1 (GeneID 223082).
Additional Product Information
Zinc- and RING- finger 2 (ZNRF2) is suggested to play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8NHG8].
Alternate Names
E3 ubiquitin-protein ligase ZNRF2; protein Ells2; RING finger protein 202; RING-type E3 ubiquitin transferase ZNRF2; RNF202; zinc and ring finger 2, E3 ubiquitin protein ligase; zinc finger/RING finger 2; zinc/RING finger protein 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.