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Rabbit anti-ZO-2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
ZO-2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 925 and 975
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Dog
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to ZO-2 immobilized on solid support.

The epitope recognized by A303-751A maps to a region between residue 900 and 950 of human Zonula Occludens Protein 2 using the numbering given in entry NP_004808.2 (GeneID 9414).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to ZO-2 immobilized on solid support.

The epitope recognized by A303-751A-T maps to a region between residue 900 and 950 of human Zonula Occludens Protein 2 using the numbering given in entry NP_004808.2 (GeneID 9414).
Country of Origin
USA

Additional Product Information

Zona occludens protein 2 (ZO-2) is a protein found in epithelial cell junctions such as tight junctions and adherens junctions.

9414
TJP2
tight junction protein 2
Q9UDY2
Tight junction protein ZO-2

Alternate Names

C9DUPq21.11; DFNA51; DUP9q21.11; Friedreich ataxia region gene X104 (tight junction protein ZO-2); PFIC4; Tight junction protein 2; tight junction protein ZO-2; X104; ZO2; zona occludens 2; Zona occludens protein 2; zonula occludens protein 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.