Sheep anti-Human Hemoglobin F Antibody HRP Conjugated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins. Sheep were immunized with purified human hemoglobin F of fetal cord blood cell origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins. Sheep were immunized with purified human hemoglobin F of fetal cord blood cell origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins. Sheep were immunized with purified human hemoglobin F of fetal red blood cell origin. Antiserum was solid phase adsorbed against hemoglobin A to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
By immunoelectrophoresis, the antiserum reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
Additional Product Information
Hemoglobin is tetrameric protein that binds iron in individual heme groups. These molecules are responsible for the binding and transport of oxygen in the blood and are the most abundant component of red blood cells. Fetal hemoglobin binds oxygen with a higher affinity than adult hemoglobin.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).
Optimal working dilutions should be determined experimentally by the investigator.