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F(ab')2 Goat anti-Mouse IgM Heavy Chain Antibody FITC Conjugated
Bethyl Laboratories
Catalog #
Target:
Mouse IgM Heavy Chain
Reactivity:
Mouse
Applications:
ELISA,
Flow Cyt,
ICC,
IF,
IHC,
WB
Host:
Goat
Clonality:
Polyclonal
Format:
F(ab')2
Isotype:
IgG
Conjugate:
Biotin,
FITC,
HRP,
R-Phycoerythrin,
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
Biotin (0.5 mg)
FITC (0.5 mg)
HRP (0.5 mg)
R-Phycoerythrin (0.5 mg)
$129.00
$169.00
Product Details
Specifications
Verified Reactivity
Mouse
Antigen Species
Mouse
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to R-phycoerythrin (RPE).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure class specificity. The antibody to mouse IgM was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to R-phycoerythrin (RPE).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species.
Country of Origin
USA
Additional Product Information
IgM is found primarily in serum and is the first antibody made to fight infections. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain.
Applications
Not all listed applications have been specifically tested by our laboratory.