F(ab')2 Goat anti-Rat IgG-Fc Fragment Cross-Adsorbed Antibody DyLight® 650 Conjugated

Name: F(ab')2 Goat anti-Rat IgG-Fc Fragment Cross-Adsorbed Antibody
Brand: Bethyl Laboratories
SKU: A110-239D5
Price: 181 USD

Bethyl Laboratories Catalog #

Target:

Rat IgG-Fc Fragment

Reactivity:

Rat

Applications:

, , ,

Host:

Goat

Clonality:

Polyclonal

Format:

F(ab')2

Isotype:

IgG

Conjugate:

DyLight® 650, R-Phycoerythrin

Purity:

Antigen Affinity Purified

DyLight® 650 (0.5 mg)

R-Phycoerythrin (0.5 mg)

$181.00 $197.00

Qty:

Product Details

Specifications

Verified Reactivity

Rat

Antigen Species

Rat

Minimal Reactivity

Human, Mouse

Concentration

0.5 mg/ml

Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State

Liquid

Buffer

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Production Procedures

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules were removed. Fragments were conjugated to R-phycoerythrin (RPE).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.

Country of Origin

USA

Additional Product Information

IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.

Applications

Not all listed applications have been specifically tested by our laboratory. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.