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Goat anti-Horse IgM Heavy Chain Antibody HRP Conjugated
Bethyl Laboratories
Catalog #
Target:
Horse IgM Heavy Chain
Reactivity:
Horse
Applications:
ELISA,
ICC,
IF,
IHC,
WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
FITC,
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Product Details
Specifications
Verified Reactivity
Horse
Antigen Species
Horse
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change
Production Procedures
Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with horse IgM. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with horse IgM. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with horse IgM. This antibody may cross react with IgM from other species.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with horse IgM. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with horse IgM. This antibody may cross react with IgM from other species. Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with horse IgM. This antibody may cross react with IgM from other species.
Country of Origin
USA
Additional Product Information
IgM is found primarily in serum and is the first antibody made to fight infections. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain.
Applications
Not all listed applications have been specifically tested by our laboratory.