Rabbit anti-Donkey IgG Heavy and Light Chain Antibody Biotinylated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Serum containing 0.09% Sodium Azide
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Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. By immunoelectrophoresis, the antiserum reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
This antibody may cross react with IgG from other species. The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 680.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 800.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. The antibody to donkey IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 755.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with donkey IgG and with light chains common to other donkey immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Additional Product Information
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).
Optimal working dilutions should be determined experimentally by the investigator.
DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 680 is excited at 682 (in PBS) and emits at 715 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 800 is excited at 770 (in PBS) and emits at 794 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 755 is excited at 754 (in PBS) and emits at 776 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.