From the latest mRNA vaccines to drug delivery systems, nanoparticles are increasingly popular therapeutic tools. Nanoparticles are versatile nano-sized vessels that shuttle small-molecule drugs, peptides, proteins, and nucleic acids to targeted sites in the body. For many scientists, how to leverage this cutting-edge technology remains a mystery. In this roundtable discussion, a panel of experts will discuss key considerations for manufacturing nanoparticle therapeutics.